Dansyl-L-proline is widely used as a chiral derivatizing agent in the separation and analysis of enantiomers in various compounds. It is particularly effective in high-performance liquid chromatography (HPLC) and fluorescence spectroscopy due to its strong fluorescence properties. This chemical aids in the determination of enantiomeric purity of amino acids and other chiral molecules, making it valuable in pharmaceutical research and quality control. Additionally, it is employed in studying enzyme kinetics and mechanisms, as it can interact with active sites of enzymes, providing insights into their function and structure. Its application extends to probing protein-ligand interactions, contributing to the development of new drugs and therapeutic agents.