L-2,4-Diaminobutyric acid hydrochloride is primarily used in biochemical research and pharmaceutical development. It serves as a precursor or intermediate in the synthesis of various biologically active compounds, including antibiotics and enzyme inhibitors. Its role in studying amino acid metabolism and transport mechanisms is significant, as it helps researchers understand cellular processes and develop targeted therapies. Additionally, it is utilized in the production of peptides and peptidomimetics, which are essential in drug discovery and development for treating diseases such as cancer and infections. Its application extends to neuroscience research, where it is used to investigate neurotransmitter systems and potential treatments for neurological disorders.