3-(2-Naphthyl)-D-alanine is primarily utilized in the field of peptide synthesis and biochemical research. It serves as a non-natural amino acid, often incorporated into peptide chains to study protein structure, function, and interactions. Its unique naphthyl group provides a hydrophobic and aromatic character, making it valuable for probing hydrophobic binding sites or enhancing peptide stability. Additionally, it is used in the development of peptide-based drugs and biomaterials, where its distinct properties can influence bioavailability, target specificity, and molecular recognition. In enzyme studies, it acts as a substrate or inhibitor to explore enzymatic mechanisms and kinetics. Its role in fluorescence-based assays is also noteworthy, as the naphthyl group can contribute to optical properties useful in detecting and tracking biological processes.