D-2,4-Diaminobutyric acid hydrochloride is primarily used in biochemical research as a building block for peptide synthesis. It serves as a precursor in the preparation of specialized peptides and amino acid derivatives, which are essential for studying protein structure and function. Additionally, it finds application in the development of enzyme inhibitors and receptor ligands, aiding in the exploration of metabolic pathways and cellular processes. Its role in medicinal chemistry is significant, particularly in the design of compounds with potential therapeutic effects, such as antimicrobial or anticancer agents. The compound is also utilized in the study of amino acid metabolism and transport mechanisms, contributing to advancements in biochemistry and molecular biology.