IT1t dihydrochloride is primarily used in research settings as a selective antagonist for the CXCR4 receptor. Its application is significant in studying the role of CXCR4 in various biological processes, including immune response, cancer metastasis, and stem cell migration. Researchers utilize this compound to block CXCR4 signaling pathways, enabling them to investigate the receptor's function in disease progression and potential therapeutic targets. Additionally, IT1t dihydrochloride is employed in experimental models to explore its potential in inhibiting HIV entry into cells, as CXCR4 serves as a co-receptor for certain strains of the virus. Its precise mechanism of action makes it a valuable tool in pharmacological and biomedical studies.