CDP-Star is widely used as a chemiluminescent substrate in molecular biology applications, particularly for the detection of alkaline phosphatase in immunoassays and nucleic acid hybridization. Its ability to produce a bright, sustained light emission upon enzymatic dephosphorylation makes it highly effective for sensitive detection in techniques such as Western blotting, Southern blotting, and Northern blotting. The chemiluminescent signal generated by CDP-Star can be captured on X-ray film or imaging systems, allowing for precise and quantitative analysis of target proteins or nucleic acids. Its stability and high signal-to-noise ratio make it a preferred choice for researchers requiring reliable and reproducible results in low-concentration samples. Additionally, it is often used in diagnostic assays for its ability to provide clear and measurable outcomes in clinical and research settings.