Coelenterazine is widely used in bioluminescence studies due to its ability to emit light when it reacts with oxygen in the presence of the enzyme luciferase. It is a key component in various bioluminescent assays, particularly in molecular biology and biomedical research. Researchers utilize it to track cellular processes, monitor gene expression, and study protein-protein interactions in live cells or organisms. Its application extends to in vivo imaging, where it helps visualize and measure biological activities in real time. Additionally, coelenterazine is employed in environmental monitoring to detect and quantify specific organisms or pollutants in water samples. Its sensitivity and versatility make it a valuable tool in both laboratory and field studies.