Luminol is widely used in forensic science for detecting trace amounts of blood at crime scenes. When sprayed on suspected areas, it reacts with the iron present in hemoglobin, producing a blue glow that is visible in the dark. This chemiluminescence helps investigators identify bloodstains that are otherwise invisible to the naked eye. Additionally, it is employed in biochemical research to study oxidative processes and detect reactive oxygen species in biological systems. In medical diagnostics, luminol is utilized in certain assays to measure peroxidase activity, aiding in the detection of infections or inflammatory conditions. Its ability to emit light upon chemical reaction also makes it valuable in educational demonstrations to illustrate principles of chemiluminescence.