Isoluminol is widely used in chemiluminescence applications, particularly in biochemical assays and diagnostic tests. It serves as a luminescent substrate for detecting peroxidase activity, making it valuable in immunoassays like ELISA (Enzyme-Linked Immunosorbent Assay) to identify specific proteins or antibodies. Its ability to emit light upon oxidation is also utilized in forensic science for detecting bloodstains, even in diluted or aged samples. Additionally, isoluminol is employed in research to study oxidative stress and reactive oxygen species (ROS) in biological systems, providing insights into cellular damage and disease mechanisms. Its sensitivity and versatility make it a key tool in both clinical and scientific settings.