Used primarily in bioluminescence imaging, it serves as a substrate for luciferase enzymes, enabling the visualization of biological processes in live organisms. This application is crucial in medical research, particularly in tracking tumor growth, monitoring gene expression, and studying infection dynamics. Additionally, it plays a significant role in drug development, where it helps in assessing the efficacy of new therapeutic compounds by providing real-time, non-invasive monitoring of cellular activity. Its ability to produce light upon enzymatic reaction makes it invaluable in various scientific studies that require precise and sensitive detection methods.