N-(4-Aminobutyl)-N-ethylisoluminol is widely used in chemiluminescence assays due to its ability to emit light upon oxidation. It is particularly valuable in immunoassays and biosensors, where it serves as a sensitive marker for detecting biological molecules. The compound is often employed in clinical diagnostics to measure substances like hormones, antibodies, or antigens in biological samples. Its high sensitivity and low background noise make it ideal for applications requiring precise detection limits. Additionally, it is utilized in environmental monitoring to detect pollutants or toxins in water and air samples. The chemiluminescent properties of this compound also find applications in research, particularly in studying oxidative stress and reactive oxygen species in biological systems. Its versatility and reliability have established it as a key component in various analytical and diagnostic tools.