Used extensively in bioluminescence imaging to study biological processes in live organisms. It serves as a substrate for luciferase enzymes, producing light when oxidized, which is crucial for tracking gene expression, tumor growth, and cellular activity in research. Widely applied in drug discovery and molecular biology to monitor real-time cellular and molecular interactions. Also utilized in environmental monitoring to detect microbial contamination and in forensic science for visualizing bloodstains. Its ability to generate measurable light makes it a key tool in non-invasive imaging techniques.