CycLuc1 is widely used in bioluminescence imaging, particularly in live-cell and in vivo studies. It serves as a substrate for luciferase enzymes, enabling the visualization and tracking of biological processes in real time. Its application is crucial in cancer research, where it helps monitor tumor growth and metastasis. Additionally, it is employed in drug discovery to assess the efficacy of therapeutic compounds by measuring cellular responses. CycLuc1’s enhanced stability and brightness make it a preferred choice for sensitive and long-term imaging experiments. It is also utilized in neuroscience to study brain activity and neural circuits, providing insights into neurological disorders.