Furimazine is widely used in bioluminescence assays, particularly in the detection of luciferase activity. It serves as a substrate for NanoLuc luciferase, enabling highly sensitive and quantitative measurement of gene expression, protein-protein interactions, and cellular signaling pathways. Its application is prominent in drug discovery, where it helps screen potential therapeutic compounds by monitoring biological responses in real-time. Additionally, it is utilized in molecular imaging to track biological processes in live cells and animal models, providing insights into disease mechanisms and treatment efficacy. Furimazine's stability and bright luminescence make it a valuable tool in both research and diagnostic applications.