Luminol Monosodium Salt is widely used in forensic science for detecting trace amounts of blood at crime scenes. When mixed with an oxidizing agent, it reacts with the iron in hemoglobin to produce a blue glow, known as chemiluminescence, which helps investigators identify bloodstains even if they are not visible to the naked eye.

In biochemistry, it serves as a chemiluminescent reagent for detecting reactive oxygen species and studying oxidative stress in biological systems. It is also employed in immunoassays and DNA detection techniques due to its ability to emit light under specific conditions.

Additionally, it is used in analytical chemistry for the detection of metal ions, particularly copper and iron, in various samples. Its sensitivity and specificity make it a valuable tool in both research and practical applications.