Luminol is widely used in forensic science for detecting trace amounts of blood at crime scenes. When mixed with an oxidizing agent, it reacts with the iron in hemoglobin to produce a blue glow, known as chemiluminescence, which helps investigators identify bloodstains even if they have been cleaned or are not visible to the naked eye. In addition to forensic applications, luminol is utilized in biological research to study cellular processes involving reactive oxygen species. It helps detect and measure oxidative stress in cells, providing insights into diseases and cellular damage. Luminol is also employed in environmental monitoring to detect the presence of certain metals, such as copper and iron, in water samples. Its sensitivity to these metals makes it a useful tool for assessing water quality and contamination levels. Furthermore, it is used in educational demonstrations to showcase chemiluminescence, making it a popular choice for teaching chemical reactions and light emission in classrooms.