Used in chemiluminescent assays for detecting reactive oxygen species in biological systems. Its high sensitivity allows for the measurement of low-level oxidative bursts in cells, such as neutrophils and macrophages during immune responses. Commonly employed in immunoassays and blotting techniques where it acts as a detection reagent when combined with peroxidase enzymes and hydrogen peroxide. Provides a stable light signal ideal for imaging and quantitative analysis in clinical diagnostics and research settings. Also utilized in forensic science for latent bloodstain detection due to its ability to emit light in the presence of heme compounds.