Widely used as a chemiluminescent reagent, this compound serves as a key component in analytical and diagnostic assays where high sensitivity detection is required. It emits light upon oxidation in the presence of catalysts such as peroxidase enzymes, making it ideal for immunoassays like ELISA and blotting techniques. Its strong signal and low background interference allow for reliable detection of trace amounts of biological molecules, including proteins and nucleic acids. Additionally, it is employed in forensic science and biomedical research for detecting blood or specific biomarkers due to its rapid and visible light emission. The compound’s stability and efficiency in aqueous solutions further enhance its utility in automated diagnostic systems.