Coelenterazine H is widely used in bioluminescence research due to its ability to act as a substrate for various luciferase enzymes. It is particularly valuable in molecular biology for studying gene expression, cellular processes, and protein-protein interactions. In live-cell imaging, it enables real-time visualization of biological events, making it a crucial tool in cancer research and drug development. Additionally, it is employed in environmental monitoring to detect pollutants and assess water quality. Its high sensitivity and compatibility with different luciferases make it a versatile compound in both academic and industrial research settings.