Used primarily as a luminescent probe in biochemical research, it is effective in detecting and quantifying reactive oxygen species (ROS) in various biological systems. Its application extends to studying oxidative stress mechanisms in cells, providing insights into the role of ROS in diseases like cancer and neurodegenerative disorders. Additionally, it serves as a sensitive marker in chemiluminescence assays, aiding in the analysis of antioxidant activity in pharmaceuticals and food products. Its stability and high quantum yield make it a valuable tool in fluorescence microscopy for visualizing cellular processes.