UV-VIS Ectoin Content Measurement
- Product Code: 35470
UV-VIS Ectoin Content Measurement
timeline Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
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1
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Collect blood... |
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Clear serum supernatant free of hemolysis or lipemia.
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2
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Add 100... |
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Two-phase separation with organic layer containing extracted tocopherols.
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3
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Transfer hexane... |
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Sample ready for chromatographic analysis.
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4
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Perform HPLC... |
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Chromatogram with separated alpha-tocopherol peak; quantify via peak area ratio to internal standard against calibration curve.
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5
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Calculate concentration... |
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Quantitative result within reference range (5.5-18.0 mg/L for adults).
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