This compound is primarily utilized in the field of biochemistry and molecular biology as a buffering agent. It helps maintain a stable pH environment in various biochemical reactions, particularly in protein purification and electrophoresis processes. Its structure, containing both amino and carboxyl groups, makes it effective in chelating metal ions, which is beneficial in preventing metal-catalyzed degradation of sensitive biological molecules. Additionally, it is used in the preparation of cell culture media to support the growth and maintenance of cells under controlled conditions. Its buffering capacity and compatibility with biological systems make it a valuable component in research and industrial applications involving enzymes, proteins, and nucleic acids.