Tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7-tricarboxylate is primarily used as an intermediate in the synthesis of macrocyclic compounds, particularly in the development of chelating agents. These chelating agents are essential in various fields, including medicinal chemistry, where they are employed to create metal complexes for diagnostic imaging, such as MRI contrast agents. The compound's structure allows it to bind selectively to metal ions, making it valuable in the design of catalysts for organic transformations. Additionally, it finds applications in environmental chemistry for the removal of heavy metals from solutions, contributing to water purification processes. Its role in coordination chemistry is also significant, aiding in the study of metal-ligand interactions and the development of new materials with specific electronic or magnetic properties.