4,4-Dimethoxychalcone is primarily studied for its potential anti-aging and cytoprotective properties. It has been shown to activate cellular stress resistance pathways, particularly those involving autophagy, which helps cells remove damaged components and may contribute to longevity. This compound exhibits antioxidant activity by reducing oxidative stress, making it of interest in research related to age-related diseases.

It also demonstrates anti-inflammatory effects by modulating signaling pathways such as NF-κB, suggesting possible applications in managing chronic inflammatory conditions. Preliminary studies indicate 4,4-Dimethoxychalcone may have anticancer potential by inducing apoptosis and inhibiting proliferation in certain cancer cell lines.

Due to its ability to mimic some effects of dietary restriction—a known lifespan extender—it is being explored as a nutraceutical or pharmacological agent for promoting healthspan. Its natural occurrence in some plants adds to its appeal for use in functional foods or supplements aimed at improving metabolic health and resilience to stress.