Azure B is widely used in biological staining, particularly for microscopy. It helps in differentiating cellular components, making it easier to study cell structures under a microscope. The dye is often employed in histology to stain nucleic acids, such as DNA and RNA, which is crucial for research in genetics and molecular biology. Additionally, Azure B is utilized in medical diagnostics to identify abnormalities in tissue samples, aiding in the detection of diseases. Its ability to bind selectively to specific cellular elements makes it a valuable tool in both research and clinical settings.