Used primarily as a reference standard in forensic toxicology and pharmaceutical research, alpha-Hydroxyalprazolam solution aids in the identification and quantification of alprazolam metabolites in biological samples. It is essential in drug metabolism studies, helping researchers understand the breakdown and elimination of alprazolam in the body. Laboratories use this solution in HPLC and LC-MS/MS methods to calibrate instruments and validate analytical procedures. Its presence supports accurate detection of benzodiazepine use in clinical and legal settings, including drug testing and overdose investigations.