Used as a reference standard in pharmaceutical and forensic analysis to identify and quantify warfarin metabolism in biological samples. Plays a role in studying drug interactions and metabolic pathways, particularly in liver enzyme studies involving CYP2C9 and CYP3A4. Helps in assessing individual patient response to warfarin therapy due to variations in metabolism. Also utilized in developing analytical methods like HPLC and mass spectrometry for monitoring anticoagulant levels and detecting metabolites in clinical and research settings.