Widely used in chemotherapy, this compound is effective in treating various types of cancer, including testicular, ovarian, bladder, lung, and head and neck cancers. It works by interfering with DNA replication in cancer cells through forming crosslinks, leading to cell death. Due to its ability to form crosslinks in DNA, it disrupts the growth and spread of malignant cells. It is often used in combination with other anticancer drugs to enhance treatment outcomes. Its application has significantly improved survival rates, especially in testicular cancer. However, its use is associated with side effects such as nephrotoxicity, ototoxicity, and nausea, which require careful patient monitoring during treatment.