Used topically as an antibiotic to prevent infection in minor cuts, scrapes, and burns. Commonly found in over-the-counter ointments and eye drops due to its effectiveness against Gram-positive bacteria. Often combined with other antibiotics like neomycin and polymyxin B in multi-ingredient formulations. Primarily acts by inhibiting bacterial cell wall synthesis, making it effective in skin and eye infections. Not used systemically due to potential kidney toxicity. Also used in veterinary medicine and in cell culture labs to control bacterial contamination.