Used primarily as an irreversible serine protease inhibitor in biochemical and biomedical research. It effectively inactivates enzymes by covalently modifying active site serine residues, making it valuable in protease activity studies and protein function analysis. Commonly employed in cell lysis buffers to prevent protein degradation during extraction and purification. Also utilized in labeling and tracking of active proteases in complex biological samples through activity-based protein profiling (ABPP). Its cell permeability allows for use in both in vitro and cellular assays, supporting studies in signal transduction, apoptosis, and disease mechanisms involving proteolytic activity.