Ala-Gln is widely used in cell culture and biopharmaceutical manufacturing as a stable dipeptide source of glutamine. Glutamine is essential for cell growth and protein synthesis but degrades easily in solution, forming toxic byproducts. Ala-Gln provides a more stable alternative that cells can efficiently utilize, helping maintain pH stability and prolonging media shelf life. It is especially valuable in large-scale mammalian cell cultures, such as those used to produce monoclonal antibodies and recombinant proteins. Its use improves cell viability and productivity, making it a preferred supplement in fed-batch processes. Additionally, Ala-Gln is used in clinical nutrition formulations for patients requiring parenteral feeding, offering a safe and effective way to deliver nitrogen and support protein metabolism.