Used in microbiology and pharmaceutical testing to determine the potency and purity of bacitracin in antibiotic formulations. Commonly applied in quality control labs for calibration of analytical instruments such as HPLC and spectrophotometers. Ensures accurate measurement in antimicrobial susceptibility testing and supports standardization in production of topical ointments and veterinary medicines. Also utilized in research settings to study antibiotic resistance and mode of action against gram-positive bacteria.