HEPES
biotech grade
Reagent
Code: #126967
Alias
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES); Hydroxyethylpiperazineethanesulfonic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, N -(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
CAS Number
7365-45-9
science Other reagents with same CAS 7365-45-9
blur_circular Chemical Specifications
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Molecular Information
Weight
238.3 g/mol
Formula
C₈H₁₈N₂O₄S
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Registry Numbers
EC Number
230-907-9
MDL Number
MFCD00006158
thermostat
Physical Properties
Melting Point
234-238 °C
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Storage & Handling
Density
1.07 g/mL at 20 °C
Storage
room temperature
description Product Description
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biological processes. HEPES is also employed in protein studies, electrophoresis, and molecular biology experiments to ensure consistent pH levels, which is critical for maintaining protein stability and activity. Additionally, it is used in microscopy and imaging techniques to stabilize pH during live-cell imaging, ensuring accurate and reliable results. Its effectiveness in a wide pH range (6.8–8.2) makes it a versatile choice for various laboratory applications.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| As | 0-0.1 ppm |
| Ca | 0-10 |
| Co | 0-5 ppm |
| Cu | 0-5 ppm |
| Fe | 0-5 ppm |
| Ignition Residue (As Sulfate) | 0-0.001 |
| K | 0-50 ppm |
| Li | 0-5 ppm |
| Loss on Drying | 0-0.005 |
| Mg | 0-5 |
| Mo | 0-5 ppm |
| Na | 0-50 |
| Pb | 0-5 |
| pH (25°C, 1 M in H2O) | 5-6.5 |
| Purity (T) | 0.995-1.005 |
| Sulfate (SO42-) | 0-50 ppm |
| Useful pH Range | 6.8-8.2 |
| UV Absorption (λ: 260 Nm, A(0.5 M in H2O)) | 0-0.1 |
| UV Absorption Λ: 280 Nm A(0.5 M In H2o) | 0-0.1 |
| Insoluble Matter (Filter Test) | Passes Filter Test |
| Proteases | None Detected |
| Solubility (H2O) | Soluble in 1 M at 20°C, clear, colorless |
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