HEPES
99%
science Other reagents with same CAS 7365-45-9
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description Product Description
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell cultures and enzymatic reactions. It is particularly effective in maintaining physiological pH levels (around 7.2–7.5) in cell culture media, making it ideal for growing mammalian cells. HEPES is also utilized in protein studies, as it minimizes interference with biochemical processes and does not form complexes with metal ions. Additionally, it is commonly employed in electrophoresis, chromatography, and other laboratory techniques where precise pH control is critical. Its low toxicity and compatibility with various biological systems make it a preferred choice in molecular biology and tissue culture applications.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Purity (Titration with NaOH) (%) | 99-101% |
| UV Absorbance (1 Mol/L, H2O, 250 nm) | 0-0.25 |
| UV Absorbance (1 Mol/L, H2O, 260 Nm) | 0-0.15 |
| UV Absorbance (1 mol/L, H2O, 320 nm) | 0-0.1 |
| Water (Karl Fischer, %) | 0-0.1 |
| Melting Point (°C) | 234-238 |
| Appearance | White powder or crystals |
| Infrared Spectrum | Conforms to Structure |
| Solubility in Water | Clear (17.5 g/100 mL) |
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