N,N'-Dicyclohexylurea (DCU) is primarily recognized as a byproduct formed during dicyclohexylcarbodiimide (DCC)-mediated peptide coupling reactions in organic synthesis, where it precipitates and is easily removed by filtration. Beyond its role as a waste product, DCU serves as a versatile intermediate in the synthesis of pharmaceuticals, agrochemicals, and other fine chemicals. It is utilized in the preparation of carbodiimides through dehydration processes and contributes to crosslinking in polymer chemistry to improve material durability and properties. Additionally, DCU acts as a chaotropic agent in biochemical applications and is employed in research to investigate urea-based reaction mechanisms and develop novel synthetic routes.