Used primarily in research and development within the fields of endocrinology and biochemistry to study steroid metabolism and enzyme activity. It serves as a reference standard in analytical techniques like HPLC and mass spectrometry for quantifying steroid hormones in biological samples. Applied in investigations of cytochrome P450 enzyme function, particularly CYP3A4, to understand its role in drug metabolism and hormonal regulation. Also utilized in the development of diagnostic assays for detecting steroid-related disorders and evaluating the efficacy of enzyme inhibitors in therapeutic contexts.