Used primarily as a serine protease inhibitor in biochemical research, this compound effectively blocks the activity of enzymes like trypsin, chymotrypsin, and thrombin. It is widely applied in studies involving protein purification and enzyme characterization to prevent unwanted proteolysis. Additionally, it serves as a valuable tool in cell biology for investigating protease-dependent cellular processes. Its ability to form irreversible bonds with active site serine residues makes it a critical reagent in understanding enzyme mechanisms and developing therapeutic inhibitors.