Used in immunology and biomedical research to isolate lymphocytes from whole blood or other biological samples. The medium enables the separation of mononuclear cells, including T cells, B cells, and NK cells, from other blood components like red blood cells and granulocytes through density gradient centrifugation. It is widely applied in clinical diagnostics, cancer research, vaccine development, and stem cell therapy due to its ability to yield high-purity, viable lymphocytes. Commonly used in both manual and automated cell separation workflows in laboratories and medical facilities.