Endotoxin-free water is crucial in various scientific and medical applications where the presence of endotoxins could compromise results or safety. It is extensively used in cell culture experiments to ensure that cells grow in a contaminant-free environment, as endotoxins can trigger immune responses and affect cell behavior. In pharmaceutical manufacturing, it is employed to prepare injectable drugs, vaccines, and other sterile products, as endotoxins can cause fever, sepsis, or other adverse reactions in patients. Additionally, it is utilized in molecular biology research for preparing reagents, buffers, and media to avoid interference with sensitive assays like PCR or protein analysis. Endotoxin-free water is also essential in medical device cleaning and rinsing to prevent contamination during surgical or diagnostic procedures. Its high purity ensures reliability and safety in critical applications across healthcare, research, and biotechnology industries.