Widely used in cell culture applications, particularly for gentle dissociation of cells without damaging cell membranes or surface proteins. Commonly applied in the isolation of intact cells from tissues, especially in primary cell culture setups. Frequently used in stem cell research, organoid culture, and tissue engineering due to its ability to cleave basement membrane proteins like fibronectin and collagen IV. Also utilized in the separation of epidermal and dermal layers in skin tissue for graft preparation. Preferred over trypsin in certain applications because it preserves cell viability and surface markers critical for downstream analysis. Used in some protocols for embryoid body formation and in the passaging of sensitive cell types such as keratinocytes and epithelial cells.