CHIR-98014 is widely used in stem cell research for maintaining pluripotency in embryonic stem cells and induced pluripotent stem cells. It functions as a potent inhibitor of GSK-3α and GSK-3β, which are key regulators in the Wnt signaling pathway. By inhibiting these kinases, CHIR-98014 promotes the stabilization of β-catenin, leading to activation of Wnt target genes that support self-renewal and prevent spontaneous differentiation. It is commonly included in culture media formulations for feeder-free stem cell cultures and is instrumental in protocols for directed differentiation, particularly in neural and cardiac lineage induction. Additionally, it has been used in reprogramming somatic cells to pluripotent states, enhancing efficiency when combined with other small molecules. Its role in modulating key signaling pathways makes it a valuable tool in regenerative medicine and drug discovery research.