Used in biopharmaceutical production to support the growth and protein expression of Chinese Hamster Ovary (CHO) cells in fed-batch culture systems. This feeding medium is optimized to enhance cell viability, increase cell density, and prolong culture duration, leading to higher yields of therapeutic proteins such as monoclonal antibodies. It typically contains concentrated nutrients, amino acids, vitamins, and energy sources that are gradually fed into the bioreactor to prevent metabolic stress and minimize the accumulation of inhibitory byproducts like lactate and ammonia. The formulation supports consistent product quality and is critical in large-scale manufacturing of biologics under controlled conditions.