Used extensively in plant tissue culture, B5 Medium supports the growth and development of plant cells and tissues in vitro. It is particularly effective for the cultivation of suspension cultures and callus tissues. The medium is favored for its balanced composition of macro and micronutrients, which promotes efficient nitrogen assimilation, especially from nitrate and ammonium sources. It is commonly used in protoplast culture, somatic embryogenesis, and genetic transformation studies. B5 Medium also supports the growth of legume cell cultures and has been adapted for use in hairy root cultures and metabolic studies. Its formulation enhances cell division and biomass accumulation, making it a preferred choice in plant biotechnology and breeding programs.