B5 Medium is widely used in plant tissue culture for the growth and maintenance of plant cells, tissues, and organs. It supports the development of callus cultures, embryo cultures, and protoplasts. The medium is especially effective for cultivating legumes and other dicotyledonous plants. It provides essential nutrients that promote cell division and differentiation, making it ideal for micropropagation and genetic transformation studies. B5 Medium is also used in the production of secondary metabolites and in research involving plant biochemistry and physiology. Its balanced composition minimizes toxicity and enhances the growth of sensitive plant species.