This medium is widely used in cell culture applications, particularly for growing hybridomas, hematopoietic cells, and other fastidious cell types. Its formulation supports optimal growth conditions by providing essential nutrients, amino acids, and vitamins. The inclusion of L-glutamine ensures a stable energy source, while sodium pyruvate aids in cellular metabolism. The HEPES buffer maintains a consistent pH, which is crucial for maintaining cell viability and function. It is commonly employed in research laboratories for studying cell behavior, drug screening, and vaccine development. Additionally, it is suitable for both suspension and adherent cell cultures, making it versatile for various experimental setups.