This medium is widely used in cell culture for growing a variety of mammalian cells, particularly in research and biopharmaceutical applications. Its high glucose content provides an energy source for cells with high metabolic demands, such as cancer cells or rapidly dividing cells. The inclusion of L-glutamine supports protein synthesis and cell growth, while the HEPES buffer helps maintain a stable pH in the culture environment, especially in open or non-CO2-regulated systems. This formulation does not contain sodium pyruvate, allowing flexibility for researchers to add it or other supplements as needed for specific protocols. It is commonly employed in experiments involving cell proliferation, transfection, and protein production. Additionally, it serves as a base medium for developing specialized formulations tailored to specific cell types or research needs. Its versatility and reliability make it a staple in laboratories focused on cell biology, drug discovery, and regenerative medicine.