This media is widely used in cell culture applications, particularly for growing a variety of mammalian cells. It is suitable for both primary and established cell lines, providing a balanced nutrient environment that supports cell growth and maintenance. The inclusion of L-glutamine ensures an essential energy source for cells, while the HEPES buffer helps maintain a stable pH, especially in open or CO2-limited environments. It is commonly utilized in research laboratories for experiments involving cell proliferation, differentiation, and toxicity studies. Additionally, it serves as a base medium for developing specialized formulations tailored to specific cell types or experimental needs. Its versatility makes it a staple in fields such as cancer research, stem cell studies, and drug development.