Widely used in cell culture applications, it provides essential nutrients for the growth of a variety of mammalian cells. The high glucose concentration supports energy-intensive processes, making it suitable for cancer cell lines and other fast-growing cells. Its formulation without L-glutamine and sodium pyruvate allows for customization, enabling researchers to add these components as needed for specific experiments. Commonly employed in virology, vaccine production, and stem cell research, it serves as a versatile base medium. It is also utilized in drug screening and toxicity testing, where controlled nutrient conditions are critical for accurate results.