Minimum Essential Medium with Hanks’ salts and L-glutamine is widely used in cell culture applications for growing a variety of cell types, particularly primary cells and cell lines. It provides essential nutrients, vitamins, and amino acids necessary for cell growth and maintenance. The inclusion of Hanks’ salts ensures a balanced physiological environment, making it suitable for experiments requiring stable pH and osmolarity. L-glutamine serves as an additional energy source, supporting protein synthesis and cellular metabolism. This medium is commonly employed in research areas such as virology, immunology, and drug screening, where consistent cell viability and proliferation are critical. It is also used in vaccine production and toxicity testing due to its ability to support healthy cell growth under controlled conditions.