This medium is widely used in cell culture applications to support the growth and maintenance of a variety of mammalian cells. It is particularly suitable for primary cell cultures and cell lines that require a balanced salt solution and essential nutrients. The inclusion of Hanks' salts ensures proper osmotic balance and pH stability, while L-glutamine serves as a critical energy source for cells. Non-essential amino acids further enhance cell growth by providing additional building blocks for protein synthesis. It is commonly employed in research laboratories for studying cell behavior, drug screening, and vaccine development. Its versatility makes it a preferred choice for experiments requiring consistent and reliable cell culture conditions.